[대학원 생명과학과 세미나 안내]
연사 : 이종봉 교수(포항공대 물리학과)
연제 : Single-molecule imaging of the dynamics of biomolecules
일시 : 2016년 11월 4일 (금) 오후 4시
장소 : 하나과학관 A동 109호
초청교수 : 김윤기 교수
Abstract
Although the temporal and mechanistic order of molecular events is essential for life, the downstream signaling mechanism in various biological processes has been poorly understood. However, since real-time observation of individual biomolecules and their complex going through biochemical reactions enables us to observe transient intermediate states, multiple pathways and the fluctuation of the molecular properties, this real-time single-molecule imaging is capable of elucidating the salient features of a variety of biological processes. I will present a successful story of how single-molecule studies have elucidated the mechanism of long-distance interactions during DNA mismatch repair (MMR). To initiate MMR that corrects misincorporated nucleotides, long-distance communication of MMR components on mismatched DNA is required: MutS that searches and recognizes nucleotide errors, and with MutL protein then transmits the mismatch recognition to a distant strand scission site (hundreds to thousands base pairs from the mismatch). However, the mechanism of communicating mismatch finding to a distant downstream site remains highly controversial for decades. If time is available, I will briefly introduce how single-molecule techniques have been used to understand long distance cell-to-cell communication.