[대학원 생명과학과 세미나 안내]
연사 : 임신혁 교수(기초과학연구원/포항공과대학교 융합생명공학부)
연제 : Role of gut micobiota in shaping and modulation of the host immune system
일시 : 2016년 10월 28일 (금) 오후 4시
장소 : 하나과학관 A동 109호
초청교수 : 김태성 교수
Abstract
Alteration of gut microbiota composition is associated with diverse immune disorders and restoration of dysbiosis in disease state with beneficial microorganism could confer the health benefits. As a modulator for dysbiosis Daily intake of oral probiotic preparations has been widely considered. Probiotics are nonpathogenic live microorganism that can provide a diverse health benefits on the host. Recently, many reports suggest that certain probiotic strains or mixture of them could exert potent immunomodulatory activity in diverse disorders. However, efficacy of probiotics is quite different depending on the type of strains and the amounts of doses. Definition of beneficial microorganisms (including probiotics) should be different depending on the types of diseases and heath condition of individual person. In our lab, we have been interested in developing probiotics that could suppressing hyper-immune disorders or enhancing immune system, and has developed diverse ex vivo and in vivo screening and evaluation systems. We have developed a screening system to selectively identify probiotic strains that could generate CD4+Foxp3+ regulatory T cells (iTregs).Among the strains, we found that Bifidobacterium bifidum IRT, and Lactobacillus reuteri IRT showed the highest IL-10highIl-12lowinducingcapability.Inthisstudy,wetestedwhethermonocolonizationofB. bifidum IRT, and L. reuteri IRT in germ free mouse to induce regulatory T cells (Treg) cells, and characterized immune regulatory activity in lamina propria of colon and small intestine. We found that oral feeding of B. bifidum IRT, and L. reuteri IRT significantly enhanced the generation of induced CD4+Foxp3+Helioslow Treg (iTreg) cells and upregulated CTLA4 expression. Treatment of BMDCs and CD4+ T-cells treated with B. bifidum IRT, and L. reuteri IRT or their culture supernatants produced high amount of IL-10 in TLR-2 dependent manner. Currently we are investigating effector molecules from the probiotics strains.