[대학원 생명과학과 세미나 안내]
연사 : 현재경 박사(한국기초과학지원연구원)
연제 : Cryo-electron microscopy for high resolution structural analysis of macromolecular complexes
일시 : 2016년 10월 7일 (금) 오후 4시
장소 : 하나과학관 A동 109호
초청교수 : 송현규 교수
Abstract
Transmission electron microscopy (TEM) is a versatile and powerful technique that enables direct visualization of biological samples of sizes ranging from whole cell to molecular resolution details of a protein molecule. Thanks to numerous technical breakthroughs and monumental discoveries, 3D electron microscopy (3DEM) has become an indispensable tool in the field of structural biology. In particular, development of cryo-electron microscopy (cryo-EM) and computational image processing played pivotal role for the determination of 3D structures of complex biological systems at sub-molecular resolution. In addition, recent near-atomic resolution structure determinations of protein complexes using cryo-EM single particle analysis opened up a new avenue in the field of structural biology.
In this presentation, basis of biological transmission electron microscopy and 3DEM will be introduced, especially focusing on technical advancements and practical applications. Also, technical specifications and the functionalities of high-end TEM instrument that was recently installed in Korea Basic Science Institute will be introduced. Finally, future prospective of constantly evolving 3DEM field will be discussed, with an anticipation of great biological discoveries that were once considered impossible.