내용요약(Abstract)
Signaling networks in mammalian cells are built from thousands of signaling proteins that carry
out a variety of cellular tasks including development, cell proliferation, cell migration, and cancer
cell metastasis. In order to understand complex cell signaling networks in mammalian cells we use
live cell imaging approaches, including visualizing behaviors and molecular interactions of signaling
proteins. We have been developing new bioimaging technologies for visualizing P-P interactions
and technologies for controlling signaling proteins in live mammalian cells. Recently, the advent of
light responsive elements in cell biology has led us to design a rapid and reversible control system
to modulate protein functions by stimulating specific wavelength of light in a spatiotemporal
manner. We expect this versatile platform could specifically control the functions of endogenous
target proteins in neuron and provide a powerful way to control a broad range of signaling
proteins in vivo system. I will discuss in the talk mostly novel imaging technologies we have
developed to control and visualize cell signaling and cell functions in mammalian cells by using
blue and green light receptors from plants.