[대학원 생명과학과 세미나 안내]
연사 : 정가영 교수 (성균관대 약학대학)
연제 : Time-resolved conformational analysis during GPCR-Gs coupling
일시 : 2019년 12월 13일 (금) 오후 5시
장소 : 하나과학관 A동 B131호
초청교수 : 우재성 교수
Protein-protein interactions and conformational changes of a signaling protein are major mechanisms of cellular signal transduction. To understand the precise signaling
mechanism, studies have investigated the structural mechanism of signaling proteins using various biochemical and/or biophysical techniques such as X-ray crystallography, nuclear magnetic resonance (NMR), electron microscopy, and electron paramagnetic resonance. In addition to these techniques, surface labeling mass spectrometry has been successfully used for conformational analysis of signaling proteins. Exposed or flexible regions have higher labeling rates and buried or ordered regions have lower labeling rates. Therefore, surface labeling mass spectrometry is a useful tool for studying protein-protein interaction interfaces and conformational changes during a signaling protein activation. Although surface labeling mass spectrometry does not provide 3-D structural information, it analyzes dynamic protein conformations that are difficult to be analyzed with other techniques.
GPCR signal transduction involves extensive protein-protein interactions and conformational changes of related signaling proteins. In this seminar, I will discuss the conformational mechanisms of GPCR signaling analyzed by surface labeling mass spectrometry.